Plasmid_Backbone
Part:BBa_K911006:Design
Designed by: Thomas Whittaker Group: iGEM12_Cambridge (2012-09-23)
Non-standard backbone for luciferase construct
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal XbaI site found at 2619 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2650
Illegal NheI site found at 580
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2656 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2650 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2650
Illegal suffix found at 2
Illegal XbaI site found at 47
Illegal XbaI site found at 2619 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2650
Plasmid lacks a suffix.
Illegal XbaI site found at 47
Illegal XbaI site found at 2619
Illegal XbaI site found at 2665
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 666
Illegal AgeI site found at 990 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
We did not design this sequence - however, it was chosen to assemble in as it is maintained at a low copy number, avoiding fatal toxicity.
Source
synthesis by DNA 2.0